Developing gold nanoparticle-based biosensors for simple and fast detection of Staphylococcus aureus

Trương Phước Long

Issue: Số 15 - Tháng 6 - 2023 Hóa học - Công nghệ thực phẩm

Developing gold nanoparticle-based biosensors for simple and fast detection of Staphylococcus aureus

Published: July 23, 2026

Abstract

Nanoparticles-based biosensors are emerging technologies for detection of pathogens. It is very challenging to develop nanoparticle-based biosensors for whole cells due to the larger size of cells compared with the nanoparticles. In this study, a new gold nanoparticle-based biosensor is developed to detect bacterial cells. Instead of trying to capture bacteria on the solid substrate, in this study, the DNA aptamers, which are capable of binding a target cells, are allowed to detach from the gold nanoparticle surface when interacting with bacteria on the sensor surface. This approach greatly increases the sensitivity of plasmonic biosensor. The results demonstrated that this sensor could detect S. aureus with a detection limit of ~10³ CFU/ml within 30 min. This method showed many advantages compared with conventional methods with respect to cost, simplicity and analysis time.

Keywords

DNA aptamer gold nanoparticles S. aureus

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