Study on the antioxidant activity of chive leaf extract (Allium tuberosum)

Abstract

This study evaluates the bioactive compounds and antioxidant capacity of chive leaf extracts in water, ethanol, and methanol. Phytochemicals were identified using color reactions, while antioxidant activity was assessed using the DPPH radical scavenging method. Results show that 70% ethanol extract exhibits the highest antioxidant activity (78.29%), higher than 96% ethanol (72.79%) and distilled water (30.48%), and comparable to vitamin C at concentrations of 40–50 µg/mL. The extract contains various bioactive compounds including alkaloids, flavonoids, saponins, terpenoids, coumarins, quinones, phenols, and tannins.
Keywords
Allium tuberosum antioxidant activity chive leaves

References

1.
Phạm Hùng Vân, & Phạm Thái Bình. (2013). Kháng sinh Đề kháng kháng sinh - Kỹ thuật kháng sinh đồ: Các vấn đề cơ bản thường gặp (tr. 61-63). Nhà xuất bản Y học, Hà Nội.
2.
Trần Thị Thùy Giang, Nguyễn Thị Nguyệt, Nguyễn Văn Trí, Nguyễn Thị Lệ Hồ, Vương Xuân Vân, Uông Nguyễn Đức Ninh, Phạm Minh Thu, & Cao Hữu Nghĩa. (2014). Khảo sát độ nhiễm khuẩn và khả năng kháng kháng sinh của Escherichia coli phân lập từ thực phẩm tại Viện Pasteur Thành phố Hồ Chí Minh. Tạp chí Khoa học, 61, 164-170. http://vci.vnu.edu.vn/upload/15022/pdf/576382337f8b9ad5458b45c5.pdf
3.
Trương Hà Thái, Phạm Hồng Ngân, Chu Thị Thanh Hương, & Cam Thị Thu Hà. (2017). Khả năng kháng kháng sinh của Escherichia coli và Salmonella phân lập từ trứng gia cầm bán tại một số chợ trên địa bàn thành phố Hà Nội. Tạp chí Khoa học Nông nghiệp Việt Nam, 15(6), 770-775. https://tapchi.vnua.edu.vn/wp-content/uploads/old/6-2017/8.pdf
4.
Nguyễn Khánh Thùy Linh, & Phạm Thị Hiền Thư. (2022). Nghiên cứu thành phần hóa học và hoạt tính kháng vi sinh vật của hẹ (Allium tuberosum). TNU Journal of Science and Technology, 227(10), 56-65. https://doi.org/10.34238/tnu-jst.5976
5.
Bauer, A. W., Kirby, W. M. M., Sherris, J. C., & Turck, M. (1966). Antibiotic susceptibility testing by a standardized single disk method. American Journal of Clinical Pathology, 45(4), 493-496.
6.
Burits, M., & Bucar, F. (2000). Antioxidant activity of Nigella sativa essential oil. Phytotherapy Research, 14(5), 323-328. https://doi.org/10.1002/1099-1573(200008)14:5%3C323::aid-ptr621%3E3.0.co;2-q
7.
Chandrashekar, P. M., Prashanth, K. V. H., & Venkatesh, Y. P. (2011). Isolation, structural elucidation and immunomodulatory activity of fructans from aged garlic extract. Phytochemistry, 72(2-3), 255-264. https://doi.org/10.1016/j.phytochem.2010.11.015
8.
Courts, F. L., & Williamson, G. (2015). The occurrence, fate and biological activities of C-glycosyl flavonoids in the human diet. Critical Reviews in Food Science and Nutrition, 55(10), 1352-1367. https://doi.org/10.1080/10408398.2012.694497
9.
Kumar, S., Narwal, S., Kumar, V., & Prakash, O. (2011). α-Glucosidase inhibitors from plants: A natural approach to treat diabetes. Pharmacognosy Reviews, 5(9), 19-29. https://doi.org/10.4103/0973-7847.79096
10.
Ma, Y. C., Yu, J., Wang, J. P., Cai, L., & Ding, Z. T. (2016). Chemical constituents of the roots of Allium tuberosum. Chinese Pharmaceutical Journal, 51(12), 972-975. https://doi.org/10.11669/cpj.2016.12.005
11.
Marxen, K., Vanselow, K. H., Lippemeier, S., Hintze, R., Ruser, A., & Hansen, U. P. (2007). Determination of DPPH radical oxidation caused by methanolic extracts of some microalgal species by linear regression analysis of spectrophotometric measurements. Sensors, 7(10), 2080-2095. https://doi.org/10.3390/s7102080
12.
Morah, F. N., & Otuk, M. E. (2015). Antimicrobial and anthelmintic activity of Eleusine indica. Acta .Sci. et. Intellectus, 1, 28–32. https://www.actaint.com/index.php/pub/article/view/45
13.
Nhut, P. T., An, T. N. T., Minh, L. V., Truc, T. T., & Anh, N. H. T. (2020). Phytochemical screening of Allium tuberosum Rottler ex Spreng as food spice. In IOP Conference Series: Materials Science and Engineering, 991(1), Article 012021. https://doi.org/10.1088/1757-899X/991/1/012021
14.
Oonmetta-Aree, J., Suzuki, T., Gasaluck, P., & Eumkeb, G. (2006). Antimicrobial properties and action of galangal (Alpinia galanga Linn.) on Staphylococcus aureus. LWT – Food Science and Technology, 39(10), 1214-1220. https://doi.org/10.1016/j.lwt.2005.06.015
15.
Parkavi, V., Vignesh, M., Selvakumar, K., Mohamed, J. M., & Ruby, J. J. (2012). Antibacterial activity of aerial parts of Imperata cylindrica (L.) Beauv. International Journal of Pharmaceutical Sciences and Drug Research, 4(3), 209-212. https://doi.org/10.25004/IJPSDR.2012.040308
16.
Pereira, E., Barros, L., Martins, A., & Ferreira, I. C. (2012). Towards chemical and nutritional inventory of Portuguese wild edible mushrooms in different habitats. Food Chemistry, 130(2), 394-403.
17.
Pietta, P. G. (2000). Flavonoids as antioxidants. Journal of natural products, 63(7), 1035-1042.
18.
Tang, X., Olatunji, O. J., Zhou, Y., & Hou, X. (2017). Allium tuberosum: Antidiabetic and hepatoprotective activities. Food Research International, 102, 681-689. https://doi.org/10.1016/j.foodres.2017.08.034
19.
Yadav, R. N. S., & Agarwala, M. (2011). Phytochemical analysis of some medicinal plants. Journal of Phytology, 3(12), 10-14. https://updatepublishing.com/journal/index.php/jp/article/view/2737