Deep eutectic solvent-assisted extraction of polyphenols from pink guava pomace

Abstract

This study investigates polyphenol extraction from pink guava (Psidium guajava L.) pomace using a deep eutectic solvent consisting of choline chloride and ethylene glycol (1:2) with 20% water. The optimal conditions were a solid-to-solvent ratio of 1:10 (w/v), 60°C, and 60 minutes. Ultrasound-assisted extraction at 30% amplitude for 15 minutes increased total polyphenol content to 107.615 ± 4.50 mg GAE/g, 37.25% higher than the non-ultrasound treatment and approximately 5.66 times that obtained by ultrasound-assisted ethanol extraction. SEM analysis showed that ultrasound disrupted cell structures, facilitating polyphenol release. The DES also retained extraction effectiveness after reuse cycles. These findings demonstrate the potential of combining DES with ultrasound as an efficient and environmentally friendly approach for recovering polyphenols from agricultural by-products.
Keywords
polyphenols pink guava pomace deep eutectic solvent ultrasound-assisted extraction antioxidant activity

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