Issue: Số 5 -Tháng 3 - 2022Hóa học - Công nghệ thực phẩm
Validating and using the HPLC-MS/MS method to determine acrylamide in coffee
Published: July 30, 2026
Abstract
In this study, the liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method was validated and applied to determine acrylamide in the coffee sample. Under the optimal experimental conditions, the linear ranged from 1000 µg/L to 1000 µg/L with the method detection limit (MDL) of 30 µg/L and the method recovery was from 87.1 % tới 99.4%. The relative standard deviations (RSD) for six repetitive experiments are lower than 3.54 % at different acrylamide concentrations. These merits show that the method is well adapted for quantitative analysis at trace concentration. This method was used to analyze some coffee samples collected from Ho Chi Minh City. Results showed that 74.19% of samples were presented with acrylamide in the concentration range from 97.33 to 560.48 µg/kg. 19.35% of samples were over the maximum permission level of acrylamide in coffee. The observation also showed that the deeper roasted coffee samples resulted in a higher contamination level of acrylamide. 100% of sample coffee with deep roasted were detected acrylamide, and 35.71% of samples exceeded the maximum permission level. This study shows that acrylamide contamination in roasted coffee is severe and it is an urgent task to control the food safety of coffee.
Keywords
acrylamidecoffeetoxicologyHPLC-MS/MS
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