Abstract
This study is to optimize the medium composition for synthesizing b-glucosidase (E.C. 3.2.1.21)
from Aspergillus nigerwith the use of water hyacinth (Eichhornia crassipes) as substrate. This study
explores the production of b-glucosidase from local available by - product materials. The study
finds out that the optimal medium compositions for the biosynthesis of b-glucosidase from
Aspergillus nigerare 22.8 percent water hyacinth; 1.17 percent glucose; 0.2 percent ammonium
sulphate and the optimal culture time is 16.6 hours. Under these medium compositions, the activity
of b-glucosidase increase 2.78 times compared to the initial survey level.
b-glucosidase
optimization
water hyacinth
Aspergillus niger
solid state
fermentation (SSF)