Issue: Số 24 - Tháng 8 - 2026Hóa học - Công nghệ thực phẩm
Simultaneous saccharification and fermentation of pineapple leaves (Ananas comosus) for bioethanol production
Published: September 16, 2026
Abstract
This study investigates dried pineapple leaves (Ananas comosus) as a lignocellulosic feedstock for bioethanol production by simultaneous saccharification and fermentation (SSF). The material contained 40.37 ± 0.32% cellulose, 25.71 ± 0.14% lignin, and 17.01 ± 0.02% reducing sugars. Pretreatment with 1 - 5% NaOH and H₂SO₄ was evaluated, and 4% NaOH for 24 hours was selected because it provided better lignin removal while limiting cellulose loss. SSF using cellulase and Saccharomyces cerevisiae was subsequently optimized for time, pH, temperature, enzyme loading, and yeast inoculum. The selected conditions were 48 hours, pH 5.0, 37°C, 5% enzyme, and 5% yeast inoculum. The highest ethanol concentration recorded was 2.93%. The findings demonstrate the potential of pineapple leaves as a feedstock for bioethanol production.
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