Ảnh hưởng của điều kiện trích ly đến hoạt tính kháng oxy hóa và kháng khuẩn của dịch chiết củ rau má thủy canh (Centella asiatica (L.) urban)

Tóm tắt

Nghiên cứu nhằm khảo sát ảnh hưởng của 3 yếu tố công nghệ trích ly gồm: tỷ lệ nguyên liệu/dung môi (1/10, 1/20, 1/30 g/mL), nhiệt độ trích ly (40-80°C) và thời gian trích ly (40-70 phút) đến hoạt tính kháng oxy hóa (DPPH) và khả năng kháng khuẩn đối với Escherichia coli và Staphylococcus aureus của dịch chiết củ rau má thủy canh (Centella asiatica (L.) Urban). Kết quả cho thấy, điều kiện trích ly tối ưu với tỷ lệ nguyên liệu/dung môi là 1/20 (g/mL) ở nhiệt độ 70°C trong 60 phút thu được dịch chiết có hoạt tính DPPH đạt 15,42 ± 0,19 µmol TE/g. Đồng thời, dịch chiết thể hiện hiệu quả kháng khuẩn mạnh, với đường kính vòng kháng khuẩn đạt 16,00 ± 1,00 mm đối với Escherichia coli và 18,33 ± 0,58 mm đối với Staphylococcus aureus ở nồng độ 1.000 mg/mL, tương đương với kháng sinh đối chứng Ampicillin (100 µg/mL). Kết quả nghiên cứu cung cấp cơ sở khoa học cho việc xây dựng quy trình trích ly hiệu quả, phục vụ khai thác nguồn dược liệu rau má thủy canh trong phát triển thực phẩm chức năng và dược liệu.

Từ khóa
rau má thủy canh trích ly kháng oxy hóa kháng khuẩn

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